Coding

Part:BBa_K2015002:Design

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


RADA16-I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You can amplify this fragment following two primers.
Forward: CAGTCTAGAATGCGTGCAGATGCACGCGCC(starting from XbaI site)
Reverse: CTGACTAGTTTATTAAGCGTCCGCACGGGCGTC(starting from SpeI site)


Source

gBlocks® Gene Fragments from IDT

References

[1] Liang P, Xiong J, Zhao L, Xu Y, Zhao J, Liu Q (2015) Recombinant self-assembling 16-residue peptide nanofiber scaffolds for neuronal axonal outgrowth. Eng. Life sci. 15(1): 152-158
[2] Nune M, Kumaraswamy P, Krishnan UM, Sethuraman S, (2013) Self-Assembling Peptide Nanofibrous Scaffolds for Tissue Engineering: Novel Approaches and Strategies for Effective Functional Regeneration. Curr. protein pept. sc. 14: 70-84.
[3] Reed CD, Barnard GC, Anderson EB, Klein LT, Gerngross TU (2006) Production and puriWcation of self-assembling peptides in Ralstonia eutropha. Protein expres. purif. 46: 179-188.
[4] Zhang S, Behnke RE, Zhao X, Spirio L, PuraMatrix: Self-assembling Peptide Nanofiber Scaffolds. Tissue eng.